Endocrine Abstracts

Background: It is well established that 1,25-dihydroxyvitamin D3 (1,25-D3) inhibits cell growth and induces apoptosis in numerous tumors. Increased expression of 1,25-D3 inactivating enzyme, (24-hydroxylase - CYP24A1), has been observed in numerous thyroid cancer cell lines after calcitriol treatment in vitro. We examined the expression of CYP24A1 as well as the activating 1-α-hydroxylase (CYP27B1) gene in human thyroid cancer tissue.Methods:...

It is established that somatic oncogene mutation (BRAF, NRAS, HRAS, KRAS) and gene translocations (RET/PTC, PAX8/PPAR-gamma) are associated with the development of thyroid cancer. Somatic single nucleotide polymorphisms were analyzed by LigthCycler melting method, while translocations were identified by real-time polymerase chain reaction technique. In 22 intraoperative thyroid tissue samples (11 pathologic and 11 normal). In tumorous sample 3 BRAF, 2 NRAS and one HRAS mutatio...

Objective: The CYP3A7 enzyme metabolizes some steroid hormones including dehydroepiandrosterone sulphate (DHEAS). Its expression silenced after birth. Previous study has shown that in case of CYP3A7*1C polymorphism, CYP3A7 enzyme activity persisted a higher level, resulting lower levels of DHEAS in men. The age-related decline of serum DHEAS levels is believed to contribute to osteoporosis. We hypothesized that CYP3A7*1C contribute bone loss through decreased level of serum DH...